Tobias Eilert, Eleni Kallis, Julia Nagy, Carlheinz Röcker and Jens Michaelis
Complete Kinetic Theory of FRET
J. Phys. Chem. B 2018, 122, 49, 11677-11694
doi.org/10.1021/acs.jpcb.8b07719

Thilo Dörfler, Tobias Eilert, Carlheinz Röcker, Julia Nagy, Jens Michaelis
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Journal of Visualized Experiments, 2017, 120
doi:10.3791/54782

Klapper. Y, Nienhaus, K., Röcker, C., Nienhaus, G.U.
Lipid membrandes and single ion channel recording for the advanced physics laboratora
We present an easy-to-handle, low-cost, and reliable setup to study various physical phenomena on a nanometer-thin lipid bilayer using the so-called black lipid membrane technique. The apparatus allows us to precisely measure optical and electrical properties of free-standing lipid membranes, to study the formation of single ion channels, and to gain detailed information on the ion conduction properties of these channels using statistical physics and autocorrelation analysis. The experiments are well suited as part of an advanced physics or biophysics laboratory course; they interconnect physics, chemistry, and biology and will be appealing to students of the natural sciences who are interested in quantitative experimentation.
Am.J.Phys. 82 (2014) 502-509
DOI: 10.1119/1.4849815

Lunov, O., Zablotskii, V., Syrovets, T., Röcker, C., Tron, K., Nienhaus, G.U. & Simmet, T.
Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages.
Biomaterials 32, 547-555 (2011).
[Abstract]

Jiang, X., Musyanovych, A., Röcker, C., Landfester, K., Mailänder, V. & Nienhaus, G.U.
Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells.
Nanoscale 3, 2028-2035 (2011).
[Abstract]

Riedinger, A., Zhang, F., Dommershausen, F., Röcker, C., Brandholt, S., Nienhaus, G.U., Koert, U. & Parak, W.J.
Ratiometric optical sensing of chloride ions with organic fluorophore-gold nanoparticle hybrids: a systematic study of design parameters and surface charge effects.
Small 6, 2590-2597 (2010).
[Abstract]

Lunov, O., Syrovets, T., Röcker, C., Tron, K., Nienhaus, G.U., Rasche, V., Mailänder, V., Landfester, K. & Simmet, T.
Lysosomal degradation of the carboxydextran shell of coated superparamagnetic iron oxide nanoparticles and the fate of professional phagocytes.
Biomaterials 31, 9015-9022 (2010).
[Abstract]

Lunov, O., Syrovets, T., Büchele, B., Jiang, X., Röcker, C., Tron, K., Nienhaus, G.U., Walther, P., Mailänder, V., Landfester, K. & Simmet, T.
The effect of carboxydextran-coated superparamagnetic iron oxide nanoparticles on c-Jun N-terminal kinase-mediated apoptosis in human macrophages.
Biomaterials 31, 5063-5071 (2010).
[Abstract]

Lehmann, A.D., Parak, W.J., Zhang, F., Ali, Z., Röcker, C., Nienhaus, G.U., Gehr, P. & Rothen-Rutishauser, B.
Fluorescent-magnetic hybrid nanoparticles induce a dose-dependent increase in proinflammatory response in lung cells in vitro correlated with intracellular localization.
Small 6, 753-762 (2010).
[Abstract]

Jiang, X., Weise, S., Hafner, M., Röcker, C., Zhang, F., Parak, W.J. & Nienhaus, G.U.
Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding.
J. R. Soc. Interface 7, S5-S13 (2010).
[Abstract]

Jiang, X., Röcker, C., Hafner, M., Brandholt, S., Dörlich, R.M. & Nienhaus, G.U.
Endo- and Exocytosis of Zwitterionic Quantum Dot Nanoparticles by Live HeLa Cells.
ACS nano 4, 6787-6797 (2010).
[Abstract]

Jiang, X., Dausend, J., Hafner, M., Musyanovych, A., Röcker, C., Landfester, K., Mailänder, V. & Nienhaus, G.U.
Specific effects of surface amines on polystyrene nanoparticles in their interactions with mesenchymal stem cells.
Biomacromolecules 11, 748-753 (2010).
[Abstract]

Röcker, C., Pötzl, M., Zhang, F., Parak, W.J. & Nienhaus, G.U.
A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles.
Nat. Nanotechnol. 4, 577-580 (2009).
[Abstract]

Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., Heilker, R., Nienhaus, G.U. & Wiedenmann, J.
mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures.
PLoS ONE 4, e4391 (2009).
[Abstract]

Bismuto, E., Di Maggio, E., Pleus, S., Sikor, M., Röcker, C., Nienhaus, G.U. & Lamb, D.C.
Molecular dynamics simulation of the acidic compact state of apomyoglobin from yellowfin tuna.
Proteins 74, 273-290 (2009).
[Abstract]

Tron, K., Manolov, D.E., Röcker, C., Kächele, M., Torzewski, J. & Nienhaus, G.U.
C-reactive protein specifically binds to Fcgamma receptor type I on a macrophage-like cell line.
Eur. J. Immunol. 38, 1414-1422 (2008).
[Abstract]

Röcker, C., Manolov, D.E., Kuzmenkina, E.V., Tron, K., Slatosch, H., Torzewski, J. & Nienhaus, G.U.
Affinity of C-reactive protein toward FcgammaRI is strongly enhanced by the gamma-chain.
Am. J. Pathol. 170, 755-763 (2007).
[Abstract]

Ivanchenko, S., Glaschick, S., Röcker, C., Oswald, F., Wiedenmann, J. & Nienhaus, G.U.
Two-photon excitation and photoconversion of EosFP in dual-color 4Pi confocal microscopy.
Biophys. J. 92, 4451-4457 (2007).
[Abstract]

Glaschick, S., Röcker, C., Deuschle, K., Wiedenmann, J., Oswald, F., Mailänder, V. & Nienhaus, G.U.
Axial Resolution Enhancement by 4Pi Confocal Fluorescence Microscopy with Two-Photon Excitation.
J. Biol. Phys. 33, 433-443 (2007).
[Abstract]

Owen, R.J., Heyes, C.D., Knebel, D., Röcker, C. & Nienhaus, G.U.
An integrated instrumental setup for the combination of atomic force microscopy with optical spectroscopy.
Biopolymers 82, 410-414 (2006).
[Abstract]

Nienhaus, G.U., Nienhaus, K., Hölzle, A., Ivanchenko, S., Renzi, F., Oswald, F., Wolff, M., Schmitt, F., Röcker, C., Vallone, B., Weidemann, W., Heilker, R., Nar, H. & Wiedenmann, J.
Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications.
Photochem. Photobiol. 82, 351-358 (2006).
[Abstract]

Wiedenmann, J., Vallone, B., Renzi, F., Nienhaus, K., Ivanchenko, S., Röcker, C. & Nienhaus, G.U.
Red fluorescent protein eqFP611 and its genetically engineered dimeric variants.
J. Biomed. Opt. 10, 014003 (2005).
[Abstract]

Rieger, R., Röcker, C. & Nienhaus, G.U.
Fluctuation correlation spectroscopy for the advanced physics laboratory.
Am. J. Phys. 73, 1129-1134 (2005).
[Abstract]

Ivanchenko, S., Röcker, C., Oswald, F., Wiedenmann, J. & Nienhaus, G.U.
Targeted green-to-red photoconversion of EosFP, a fluorescent marker protein.
J. Biol. Phys. 31, 249-259 (2005).
[Abstract]

Anikin, K., Röcker, C., Wittemann, A., Wiedenmann, J., Ballauff, M. & Nienhaus, G.U.
Polyelectrolyte-mediated protein adsorption: fluorescent protein binding to individual polyelectrolyte nanospheres.
J. Phys. Chem. B 109, 5418-5420 (2005).
[Abstract]

Wiedenmann, J., Vallone, B., Renzi, F., Nienhaus, K., Ivanchenko, S., Röcker, C. & Nienhaus, G.U.
Dimeric variants of the red fluorescent protein eqFP611 generated by site-directed mutagenesis.
Proc. SPIE 5329, 23-29 (2004).
[Abstract]

Wiedenmann, J., Ivanchenko, S., Oswald, F., Schmitt, F., Röcker, C., Salih, A., Spindler, K.D. & Nienhaus, G.U.
EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion.
Proc. Natl. Acad. Sci. USA 101, 15905-15910 (2004).
[Abstract]

Schenk, A., Ivanchenko, S., Röcker, C., Wiedenmann, J. & Nienhaus, G.U.
Photodynamics of red fluorescent proteins studied by fluorescence correlation spectroscopy.
Biophys. J. 86, 384-394 (2004).
[Abstract]

Manolov, D.E., Röcker, C., Hombach, V., Nienhaus, G.U. & Torzewski, J.
Ultrasensitive confocal fluorescence microscopy of C-reactive protein interacting with FcgammaRIIa.
Arterioscler. Thromb. Vasc. Biol. 24, 2372-2377 (2004).
[Abstract]

Groll, J., Amirgoulova, E.V., Ameringer, T., Heyes, C.D., Röcker, C., Nienhaus, G.U. & Möller, M.
Biofunctionalized, ultrathin coatings of cross-linked star-shaped poly(ethylene oxide) allow reversible folding of immobilized proteins.
J. Am. Chem. Soc. 126, 4234-4239 (2004).
[Abstract]

Amirgoulova, E.V., Groll, J., Heyes, C.D., Ameringer, T., Röcker, C., Möller, M. & Nienhaus, G.U.
Biofunctionalized polymer surfaces exhibiting minimal interaction towards immobilized proteins.
Chemphyschem 5, 552-555 (2004).
[Abstract]

Wiedenmann, J., Schenk, A., Röcker, C., Girod, A., Spindler, K.D. & Nienhaus, G.U.
A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria).
Proc. Natl. Acad. Sci. USA 99, 11646-11651 (2002).
[Abstract]

Chan, V.Z.H., Codd, S.L., van der Helm, M.J., Spatz, J.P., Röcker, C., Nienhaus, G.U., Levi, S., van Veggel, F.C.J.M., Reinhoudt, D.N. & Möller, M.
Sub-10 nm gold nanoarrays for tethering single molecules.
Materials Research Society Symposium Proceedings 676, Y4.4.1-Y4.4.6 (2001).
[Abstract]

Lamb, D.C., Schenk, A., Röcker, C., Scalfi-Happ, C. & Nienhaus, G.U.
Sensitivity enhancement in fluorescence correlation spectroscopy of multiple species using time-gated detection.
Biophys. J. 79, 1129-1138 (2000).
[Abstract]

Lamb, D.C., Schenk, A., Röcker, C. & Nienhaus, G.U.
Determining chemical rate coefficients using time-gated fluorescence correlation spectroscopy.
J. Phys. Org. Chem. 13, 654-658 (2000).
[Abstract]

Wiedenmann, J., Röcker, C. & Funke, W.
The morphs of Anemonia aff. Sulcata (Cnidaria, Anthozoa) in particular consideration of the ectodermal pigments.
Verhandlungen der Gesellschaft für Ökologie 29, 497-503 (1999).

Röcker, C., Heilemann, A. & Fromherz, P.
Time-resolved fluorescence of a hemicyanine dye: dynamics of rotamerism and resolvation.
J. Phys. Chem. 100, 12172-12177 (1996).
[Abstract]

Fromherz, P. & Röcker, C.
Staining of biomembranes with amphiphilic hemicyanine dyes.
Ber. Bunsenges. Phys. Chem. 98, 128-131 (1994).
[Abstract]

Fromherz, P., Röcker, C. & Rüppel, D.
From discoid micelles to spherical vesicles: the concept of edge activity.
Faraday Discuss. Chem. Soc. 81, 39-48 (1986).
[Abstract]