Publications
Here you can find some selected publications (last update: Jan 2024).
For the full publication list please refer to the PubMed site.
Tittes, C.; Nijland, J.; Schoentag, A. M. C.; Hackl, T.; Di Cianni, N.; Marchfelder, A.; Quax, T. E. F. Development of a Genetic System for Haloferax Gibbonsii LR2-5, Model Host for Haloarchaeal Viruses. Appl Environ Microbiol 2024, 90 (4), e0012924. https://doi.org/10.1128/aem.00129-24.
Schiller, H.; Hong, Y.; Kouassi, J.; Rados, T.; Kwak, J.; DiLucido, A.; Safer, D.; Marchfelder, A.; Pfeiffer, F.; Bisson, A.; Schulze, S.; Pohlschroder, M. Identification of Structural and Regulatory Cell-Shape Determinants in Haloferax Volcanii. Nat Commun 2024, 15 (1), 1414. https://doi.org/10.1038/s41467-024-45196-0.
Sailer, A.-L.; Jevtic, Z.; Stoll, B.; Wörtz, J.; Sharma, K.; Urlaub, H.; Dyall-Smith, M.; Pfeiffer, F.; Marchfelder, A.; Lenz, C. Iron Starvation Results in Up-Regulation of a Probable Haloferax Volcanii Siderophore Transporter. Front Microbiol 2024, 15, 1422844. https://doi.org/10.3389/fmicb.2024.1422844.
Mills, J.; Gebhard, L. J.; Schubotz, F.; Shevchenko, A.; Speth, D. R.; Liao, Y.; Duggin, I. G.; Marchfelder, A.; Erdmann, S. Extracellular Vesicle Formation in Euryarchaeota Is Driven by a Small GTPase. Proc Natl Acad Sci U S A 2024, 121 (10), e2311321121. https://doi.org/10.1073/pnas.2311321121.
Miezner, G.; Turgeman-Grott, I.; Zatopek, K. M.; Gardner, A. F.; Reshef, L.; Choudhary, D. K.; Alstetter, M.; Allers, T.; Marchfelder, A.; Gophna, U. An Archaeal Cas3 Protein Facilitates Rapid Recovery from DNA Damage. Microlife 2023, 4, uqad007. https://doi.org/10.1093/femsml/uqad007.
Hadjeras, L.; Bartel, J.; Maier, L.-K.; Maaß, S.; Vogel, V.; Svensson, S. L.; Eggenhofer, F.; Gelhausen, R.; Müller, T.; Alkhnbashi, O. S.; Backofen, R.; Becher, D.; Sharma, C. M.; Marchfelder, A. Revealing the Small Proteome of Haloferax Volcanii by Combining Ribosome Profiling and Small-Protein Optimized Mass Spectrometry. Microlife 2023, 4, uqad001. https://doi.org/10.1093/femsml/uqad001.
Wörtz, J.; Smith, V.; Fallmann, J.; König, S.; Thuraisingam, T.; Walther, P.; Urlaub, H.; Stadler, P. F.; Allers, T.; Hille, F.; Marchfelder, A. Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair. Front Microbiol 2022, 13, 822304. https://doi.org/10.3389/fmicb.2022.822304.
Schwarz, T. S.; Schreiber, S. S.; Marchfelder, A. CRISPR Interference as a Tool to Repress Gene Expression in Haloferax Volcanii. Methods Mol Biol 2022, 2522, 57–85. https://doi.org/10.1007/978-1-0716-2445-6_4.
Märkle, P.; Maier, L.-K.; Maaß, S.; Hirschfeld, C.; Bartel, J.; Becher, D.; Voß, B.; Marchfelder, A. A Small RNA Is Linking CRISPR-Cas and Zinc Transport. Front Mol Biosci 2021, 8, 640440. https://doi.org/10.3389/fmolb.2021.640440.
Liao, Y.; Vogel, V.; Hauber, S.; Bartel, J.; Alkhnbashi, O. S.; Maaß, S.; Schwarz, T. S.; Backofen, R.; Becher, D.; Duggin, I. G.; Marchfelder, A. CdrS Is a Global Transcriptional Regulator Influencing Cell Division in Haloferax Volcanii. mBio 2021, 12 (4), e0141621. https://doi.org/10.1128/mBio.01416-21.
Turkowyd, B.; Schreiber, S.; Wörtz, J.; Segal, E. S.; Mevarech, M.; Duggin, I. G.; Marchfelder, A.; Endesfelder, U. Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax Volcanii. Front Microbiol 2020, 11, 583010. https://doi.org/10.3389/fmicb.2020.583010.
Stachler, A.-E.; Wörtz, J.; Alkhnbashi, O. S.; Turgeman-Grott, I.; Smith, R.; Allers, T.; Backofen, R.; Gophna, U.; Marchfelder, A. Adaptation Induced by Self-Targeting in a Type I-B CRISPR-Cas System. J Biol Chem 2020, 295 (39), 13502–13515. https://doi.org/10.1074/jbc.RA120.014030.
Stachler, A.-E.; Schwarz, T. S.; Schreiber, S.; Marchfelder, A. CRISPRi as an Efficient Tool for Gene Repression in Archaea. Methods 2020, 172, 76–85. https://doi.org/10.1016/j.ymeth.2019.05.023.
Schwarz, T. S.; Berkemer, S. J.; Bernhart, S. H.; Weiß, M.; Ferreira-Cerca, S.; Stadler, P. F.; Marchfelder, A. Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea. Front Microbiol 2020, 11, 594838. https://doi.org/10.3389/fmicb.2020.594838.
Schulze, S.; Adams, Z.; Cerletti, M.; De Castro, R.; Ferreira-Cerca, S.; Fufezan, C.; Giménez, M. I.; Hippler, M.; Jevtic, Z.; Knüppel, R.; Legerme, G.; Lenz, C.; Marchfelder, A.; Maupin-Furlow, J.; Paggi, R. A.; Pfeiffer, F.; Poetsch, A.; Urlaub, H.; Pohlschroder, M. The Archaeal Proteome Project Advances Knowledge about Archaeal Cell Biology through Comprehensive Proteomics. Nat Commun 2020, 11 (1), 3145. https://doi.org/10.1038/s41467-020-16784-7.
Pfeiffer, F.; Losensky, G.; Marchfelder, A.; Habermann, B.; Dyall-Smith, M. Whole-Genome Comparison between the Type Strain of Halobacterium Salinarum (DSM 3754(T) ) and the Laboratory Strains R1 and NRC-1. Microbiologyopen 2020, 9 (2), e974. https://doi.org/10.1002/mbo3.974.
Marchfelder, A. CRISPR-Cas: In Rekordzeit von der Grundlagenforschung zur Anwendung. Biospektrum (Heidelb) 2020, 26 (7), 806. https://doi.org/10.1007/s12268-020-1500-5.
Maier, L.-K.; Marchfelder, A.; Randau, L. Meeting Report: German Genetics Society-Genome Editing with CRISPR. Bioessays 2020, 42 (2), e1900223. https://doi.org/10.1002/bies.201900223.
Kubatova, N.; Pyper, D. J.; Jonker, H. R. A.; Saxena, K.; Remmel, L.; Richter, C.; Brantl, S.; Evguenieva-Hackenberg, E.; Hess, W. R.; Klug, G.; Marchfelder, A.; Soppa, J.; Streit, W.; Mayzel, M.; Orekhov, V. Y.; Fuxreiter, M.; Schmitz, R. A.; Schwalbe, H. Rapid Biophysical Characterization and NMR Spectroscopy Structural Analysis of Small Proteins from Bacteria and Archaea. Chembiochem 2020, 21 (8), 1178–1187. https://doi.org/10.1002/cbic.201900677.
Kubatova, N.; Jonker, H. R. A.; Saxena, K.; Richter, C.; Vogel, V.; Schreiber, S.; Marchfelder, A.; Schwalbe, H. Solution Structure and Dynamics of the Small Protein HVO_2922 from Haloferax Volcanii. Chembiochem 2020, 21 (1–2), 149–156. https://doi.org/10.1002/cbic.201900085.
Berkemer, S. J.; Maier, L.-K.; Amman, F.; Bernhart, S. H.; Wörtz, J.; Märkle, P.; Pfeiffer, F.; Stadler, P. F.; Marchfelder, A. Identification of RNA 3´ Ends and Termination Sites in Haloferax Volcanii. RNA Biol 2020, 17 (5), 663–676. https://doi.org/10.1080/15476286.2020.1723328.
Turgeman-Grott, I.; Joseph, S.; Marton, S.; Eizenshtein, K.; Naor, A.; Soucy, S. M.; Stachler, A.-E.; Shalev, Y.; Zarkor, M.; Reshef, L.; Altman-Price, N.; Marchfelder, A.; Gophna, U. Pervasive Acquisition of CRISPR Memory Driven by Inter-Species Mating of Archaea Can Limit Gene Transfer and Influence Speciation. Nat Microbiol 2019, 4 (1), 177–186. https://doi.org/10.1038/s41564-018-0302-8.
Schwarz, T. S.; Wäber, N. B.; Feyh, R.; Weidenbach, K.; Schmitz, R. A.; Marchfelder, A.; Hartmann, R. K. Homologs of Aquifex Aeolicus Protein-Only RNase P Are Not the Major RNase P Activities in the Archaea Haloferax Volcanii and Methanosarcina Mazei. IUBMB Life 2019, 71 (8), 1109–1116. https://doi.org/10.1002/iub.2122.
Pfeiffer, F.; Marchfelder, A.; Habermann, B.; Dyall-Smith, M. L. The Genome Sequence of the Halobacterium Salinarum Type Strain Is Closely Related to That of Laboratory Strains NRC-1 and R1. Microbiol Resour Announc 2019, 8 (28). https://doi.org/10.1128/MRA.00429-19.
Maier, L.-K.; Stachler, A.-E.; Brendel, J.; Stoll, B.; Fischer, S.; Haas, K. A.; Schwarz, T. S.; Alkhnbashi, O. S.; Sharma, K.; Urlaub, H.; Backofen, R.; Gophna, U.; Marchfelder, A. The Nuts and Bolts of the Haloferax CRISPR-Cas System I-B. RNA Biol 2019, 16 (4), 469–480. https://doi.org/10.1080/15476286.2018.1460994.
Maier, L.-K.; Marchfelder, A. It’s All about the T: Transcription Termination in Archaea. Biochem Soc Trans 2019, 47 (1), 461–468. https://doi.org/10.1042/BST20180557.
Jevtić, Ž.; Stoll, B.; Pfeiffer, F.; Sharma, K.; Urlaub, H.; Marchfelder, A.; Lenz, C. The Response of Haloferax Volcanii to Salt and Temperature Stress: A Proteome Study by Label-Free Mass Spectrometry. Proteomics 2019, 19 (20), e1800491. https://doi.org/10.1002/pmic.201800491.
Charpentier, E.; Elsholz, A.; Marchfelder, A. CRISPR-Cas: More than Ten Years and Still Full of Mysteries. RNA Biol 2019, 16 (4), 377–379. https://doi.org/10.1080/15476286.2019.1591659.
Clouet-d’Orval, B.; Batista, M.; Bouvier, M.; Quentin, Y.; Fichant, G.; Marchfelder, A.; Maier, L.-K. Insights into RNA-Processing Pathways and Associated RNA-Degrading Enzymes in Archaea. FEMS Microbiol Rev 2018, 42 (5), 579–613. https://doi.org/10.1093/femsre/fuy016.
Stachler, A.-E.; Turgeman-Grott, I.; Shtifman-Segal, E.; Allers, T.; Marchfelder, A.; Gophna, U. High Tolerance to Self-Targeting of the Genome by the Endogenous CRISPR-Cas System in an Archaeon. Nucleic Acids Res 2017, 45 (9), 5208–5216. https://doi.org/10.1093/nar/gkx150.
Ma, M.; Li de la Sierra-Gallay, I.; Lazar, N.; Pellegrini, O.; Durand, D.; Marchfelder, A.; Condon, C.; van Tilbeurgh, H. The Crystal Structure of Trz1, the Long Form RNase Z from Yeast. Nucleic Acids Res 2017, 45 (10), 6209–6216. https://doi.org/10.1093/nar/gkx216.
Gophna, U.; Allers, T.; Marchfelder, A. Finally, Archaea Get Their CRISPR-Cas Toolbox. Trends Microbiol 2017, 25 (6), 430–432. https://doi.org/10.1016/j.tim.2017.03.009.
Stachler, A.-E.; Marchfelder, A. Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System. J Biol Chem 2016, 291 (29), 15226–15242. https://doi.org/10.1074/jbc.M116.724062.
Babski, J.; Haas, K. A.; Näther-Schindler, D.; Pfeiffer, F.; Förstner, K. U.; Hammelmann, M.; Hilker, R.; Becker, A.; Sharma, C. M.; Marchfelder, A.; Soppa, J. Genome-Wide Identification of Transcriptional Start Sites in the Haloarchaeon Haloferax Volcanii Based on Differential RNA-Seq (dRNA-Seq). BMC Genomics 2016, 17 (1), 629. https://doi.org/10.1186/s12864-016-2920-y.
Sharma, K.; Hrle, A.; Kramer, K.; Sachsenberg, T.; Staals, R. H. J.; Randau, L.; Marchfelder, A.; van der Oost, J.; Kohlbacher, O.; Conti, E.; Urlaub, H. Analysis of Protein-RNA Interactions in CRISPR Proteins and Effector Complexes by UV-Induced Cross-Linking and Mass Spectrometry. Methods 2015, 89, 138–148. https://doi.org/10.1016/j.ymeth.2015.06.005.
Maier, L.-K.; Stachler, A.-E.; Saunders, S. J.; Backofen, R.; Marchfelder, A. An Active Immune Defense with a Minimal CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) RNA and without the Cas6 Protein. J Biol Chem 2015, 290 (7), 4192–4201. https://doi.org/10.1074/jbc.M114.617506.
Maier, L.-K.; Dyall-Smith, M.; Marchfelder, A. The Adaptive Immune System of Haloferax Volcanii. Life (Basel) 2015, 5 (1), 521–537. https://doi.org/10.3390/life5010521.
Maier, L.-K.; Benz, J.; Fischer, S.; Alstetter, M.; Jaschinski, K.; Hilker, R.; Becker, A.; Allers, T.; Soppa, J.; Marchfelder, A. Deletion of the Sm1 Encoding Motif in the Lsm Gene Results in Distinct Changes in the Transcriptome and Enhanced Swarming Activity of Haloferax Cells. Biochimie 2015, 117, 129–137. https://doi.org/10.1016/j.biochi.2015.02.023.
Cass, S. D. B.; Haas, K. A.; Stoll, B.; Alkhnbashi, O. S.; Sharma, K.; Urlaub, H.; Backofen, R.; Marchfelder, A.; Bolt, E. L. The Role of Cas8 in Type I CRISPR Interference. Biosci Rep 2015, 35 (3). https://doi.org/10.1042/BSR20150043.
Skowronek, E.; Grzechnik, P.; Späth, B.; Marchfelder, A.; Kufel, J. tRNA 3’ Processing in Yeast Involves tRNase Z, Rex1, and Rrp6. RNA 2014, 20 (1), 115–130. https://doi.org/10.1261/rna.041467.113.
Jaschinski, K.; Babski, J.; Lehr, M.; Burmester, A.; Benz, J.; Heyer, R.; Dörr, M.; Marchfelder, A.; Soppa, J. Generation and Phenotyping of a Collection of sRNA Gene Deletion Mutants of the Haloarchaeon Haloferax Volcanii. PLoS One 2014, 9 (3), e90763. https://doi.org/10.1371/journal.pone.0090763.
Hrle, A.; Maier, L.-K.; Sharma, K.; Ebert, J.; Basquin, C.; Urlaub, H.; Marchfelder, A.; Conti, E. Structural Analyses of the CRISPR Protein Csc2 Reveal the RNA-Binding Interface of the Type I-D Cas7 Family. RNA Biol 2014, 11 (8), 1072–1082. https://doi.org/10.4161/rna.29893.
Brendel, J.; Stoll, B.; Lange, S. J.; Sharma, K.; Lenz, C.; Stachler, A.-E.; Maier, L.-K.; Richter, H.; Nickel, L.; Schmitz, R. A.; Randau, L.; Allers, T.; Urlaub, H.; Backofen, R.; Marchfelder, A. A Complex of Cas Proteins 5, 6, and 7 Is Required for the Biogenesis and Stability of Clustered Regularly Interspaced Short Palindromic Repeats (Crispr)-Derived Rnas (Crrnas) in Haloferax Volcanii. J Biol Chem 2014, 289 (10), 7164–7177. https://doi.org/10.1074/jbc.M113.508184.
Babski, J.; Maier, L.-K.; Heyer, R.; Jaschinski, K.; Prasse, D.; Jäger, D.; Randau, L.; Schmitz, R. A.; Marchfelder, A.; Soppa, J. Small Regulatory RNAs in Archaea. RNA Biol 2014, 11 (5), 484–493. https://doi.org/10.4161/rna.28452.
Stoll, B.; Maier, L.-K.; Lange, S. J.; Brendel, J.; Fischer, S.; Backofen, R.; Marchfelder, A. Requirements for a Successful Defence Reaction by the CRISPR-Cas Subtype I-B System. Biochem Soc Trans 2013, 41 (6), 1444–1448. https://doi.org/10.1042/BST20130098.
Schnattinger, T.; Schöning, U.; Marchfelder, A.; Kestler, H. A. RNA-Pareto: Interactive Analysis of Pareto-Optimal RNA Sequence-Structure Alignments. Bioinformatics 2013, 29 (23), 3102–3104. https://doi.org/10.1093/bioinformatics/btt536.
Marchfelder, A. Special Focus CRISPR-Cas. RNA Biol 2013, 10 (5), 655–658. https://doi.org/10.4161/rna.24687.
Maier, L.-K.; Stoll, B.; Brendel, J.; Fischer, S.; Pfeiffer, F.; Dyall-Smith, M.; Marchfelder, A. The Ring of Confidence: A Haloarchaeal CRISPR/Cas System. Biochem Soc Trans 2013, 41 (1), 374–378. https://doi.org/10.1042/BST20120263.
Maier, L.-K.; Lange, S. J.; Stoll, B.; Haas, K. A.; Fischer, S.; Fischer, E.; Duchardt-Ferner, E.; Wöhnert, J.; Backofen, R.; Marchfelder, A. Essential Requirements for the Detection and Degradation of Invaders by the Haloferax Volcanii CRISPR/Cas System I-B. RNA Biol 2013, 10 (5), 865–874. https://doi.org/10.4161/rna.24282.
Peters, K.; Niessen, M.; Peterhänsel, C.; Späth, B.; Hölzle, A.; Binder, S.; Marchfelder, A.; Braun, H.-P. Complex I-Complex II Ratio Strongly Differs in Various Organs of Arabidopsis Thaliana. Plant Mol Biol 2012, 79 (3), 273–284. https://doi.org/10.1007/s11103-012-9911-4.
Marchfelder, A.; Fischer, S.; Brendel, J.; Stoll, B.; Maier, L.-K.; Jäger, D.; Prasse, D.; Plagens, A.; Schmitz, R. A.; Randau, L. Small RNAs for Defence and Regulation in Archaea. Extremophiles 2012, 16 (5), 685–696. https://doi.org/10.1007/s00792-012-0469-5.
Maier, L.-K.; Fischer, S.; Stoll, B.; Brendel, J.; Pfeiffer, F.; Dyall-Smith, M.; Marchfelder, A. The Immune System of Halophilic Archaea. Mob Genet Elements 2012, 2 (5), 228–232. https://doi.org/10.4161/mge.22530.
Hölzle, A.; Stoll, B.; Schnattinger, T.; Schöning, U.; Tjaden, B.; Marchfelder, A. tRNA-like Elements in Haloferax Volcanii. Biochimie 2012, 94 (4), 940–946. https://doi.org/10.1016/j.biochi.2011.12.002.
Heyer, R.; Dörr, M.; Jellen-Ritter, A.; Späth, B.; Babski, J.; Jaschinski, K.; Soppa, J.; Marchfelder, A. High Throughput Sequencing Reveals a Plethora of Small RNAs Including tRNA Derived Fragments in Haloferax Volcanii. RNA Biol 2012, 9 (7), 1011–1018. https://doi.org/10.4161/rna.20826.
Fischer, S.; Maier, L.-K.; Stoll, B.; Brendel, J.; Fischer, E.; Pfeiffer, F.; Dyall-Smith, M.; Marchfelder, A. An Archaeal Immune System Can Detect Multiple Protospacer Adjacent Motifs (PAMs) to Target Invader DNA. J Biol Chem 2012, 287 (40), 33351–33363. https://doi.org/10.1074/jbc.M112.377002.
Fischer, S.; John von Freyend, S.; Sabag-Daigle, A.; Daniels, C. J.; Allers, T.; Marchfelder, A. Assigning a Function to a Conserved Archaeal Metallo-β-Lactamase from Haloferax Volcanii. Extremophiles 2012, 16 (2), 333–343. https://doi.org/10.1007/s00792-012-0433-4.
Hammann, C.; Hartmann, R. K.; Helm, M.; Klostermeier, D.; Marchfelder, A.; Suess, B.; Vörtler, S. Regulatory RNAs and Beyond. EMBO Rep 2011, 12 (8), 751–753. https://doi.org/10.1038/embor.2011.150.
Fischer, S.; Benz, J.; Späth, B.; Jellen-Ritter, A.; Heyer, R.; Dörr, M.; Maier, L.-K.; Menzel-Hobeck, C.; Lehr, M.; Jantzer, K.; Babski, J.; Soppa, J.; Marchfelder, A. Regulatory RNAs in Haloferax Volcanii. Biochem Soc Trans 2011, 39 (1), 159–162. https://doi.org/10.1042/BST0390159.
Babski, J.; Tjaden, B.; Voss, B.; Jellen-Ritter, A.; Marchfelder, A.; Hess, W. R.; Soppa, J. Bioinformatic Prediction and Experimental Verification of sRNAs in the Haloarchaeon Haloferax Volcanii. RNA Biol 2011, 8 (5), 806–816. https://doi.org/10.4161/rna.8.5.16039.
Fischer, S.; Benz, J.; Späth, B.; Maier, L.-K.; Straub, J.; Granzow, M.; Raabe, M.; Urlaub, H.; Hoffmann, J.; Brutschy, B.; Allers, T.; Soppa, J.; Marchfelder, A. The Archaeal Lsm Protein Binds to Small RNAs. J Biol Chem 2010, 285 (45), 34429–34438. https://doi.org/10.1074/jbc.M110.118950.
Straub, J.; Brenneis, M.; Jellen-Ritter, A.; Heyer, R.; Soppa, J.; Marchfelder, A. Small RNAs in Haloarchaea: Identification, Differential Expression and Biological Function. RNA Biol 2009, 6 (3), 281–292. https://doi.org/10.4161/rna.6.3.8357.
Soppa, J.; Straub, J.; Brenneis, M.; Jellen-Ritter, A.; Heyer, R.; Fischer, S.; Granzow, M.; Voss, B.; Hess, W. R.; Tjaden, B.; Marchfelder, A. Small RNAs of the Halophilic Archaeon Haloferax Volcanii. Biochem Soc Trans 2009, 37 (Pt 1), 133–136. https://doi.org/10.1042/BST0370133.
Hartmann, R. K.; Gössringer, M.; Späth, B.; Fischer, S.; Marchfelder, A. The Making of tRNAs and More - RNase P and tRNase Z. Prog Mol Biol Transl Sci 2009, 85, 319–368. https://doi.org/10.1016/S0079-6603(08)00808-8.
Canino, G.; Bocian, E.; Barbezier, N.; Echeverría, M.; Forner, J.; Binder, S.; Marchfelder, A. Arabidopsis Encodes Four tRNase Z Enzymes. Plant Physiol 2009, 150 (3), 1494–1502. https://doi.org/10.1104/pp.109.137950.
Barbezier, N.; Canino, G.; Rodor, J.; Jobet, E.; Saez-Vasquez, J.; Marchfelder, A.; Echeverría, M. Processing of a Dicistronic tRNA-snoRNA Precursor: Combined Analysis in Vitro and in Vivo Reveals Alternate Pathways and Coupling to Assembly of snoRNP. Plant Physiol 2009, 150 (3), 1598–1610. https://doi.org/10.1104/pp.109.137968.
Späth, B.; Schubert, S.; Lieberoth, A.; Settele, F.; Schütz, S.; Fischer, S.; Marchfelder, A. Two Archaeal tRNase Z Enzymes: Similar but Different. Arch Microbiol 2008, 190 (3), 301–308. https://doi.org/10.1007/s00203-008-0368-4.
Hölzle, A.; Fischer, S.; Heyer, R.; Schütz, S.; Zacharias, M.; Walther, P.; Allers, T.; Marchfelder, A. Maturation of the 5S rRNA 5’ End Is Catalyzed in Vitro by the Endonuclease tRNase Z in the Archaeon H. Volcanii. RNA 2008, 14 (5), 928–937. https://doi.org/10.1261/rna.933208.
Späth, B.; Settele, F.; Schilling, O.; D’Angelo, I.; Vogel, A.; Feldmann, I.; Meyer-Klaucke, W.; Marchfelder, A. Metal Requirements and Phosphodiesterase Activity of tRNase Z Enzymes. Biochemistry 2007, 46 (51), 14742–14750. https://doi.org/10.1021/bi7010459.
Späth, B.; Canino, G.; Marchfelder, A. tRNase Z: The End Is Not in Sight. Cell Mol Life Sci 2007, 64 (18), 2404–2412. https://doi.org/10.1007/s00018-007-7160-5.
Hammann, C.; Hartmann, R. K.; Marchfelder, A. 25 Years of Catalytic RNA: Looking Younger than Ever! Biol Chem 2007, 388 (7), 659–660. https://doi.org/10.1515/BC.2007.086.
Vogel, A.; Schilling, O.; Späth, B.; Marchfelder, A. The tRNase Z Family of Proteins: Physiological Functions, Substrate Specificity and Structural Properties. Biol Chem 2005, 386 (12), 1253–1264. https://doi.org/10.1515/BC.2005.142.
Späth, B.; Kirchner, S.; Vogel, A.; Schubert, S.; Meinlschmidt, P.; Aymanns, S.; Nezzar, J.; Marchfelder, A. Analysis of the Functional Modules of the tRNA 3’ Endonuclease (tRNase Z). J Biol Chem 2005, 280 (42), 35440–35447. https://doi.org/10.1074/jbc.M506418200.
Schilling, O.; Vogel, A.; Kostelecky, B.; Natal da Luz, H.; Spemann, D.; Späth, B.; Marchfelder, A.; Tröger, W.; Meyer-Klaucke, W. Zinc- and Iron-Dependent Cytosolic Metallo-Beta-Lactamase Domain Proteins Exhibit Similar Zinc-Binding Affinities, Independent of an Atypical Glutamate at the Metal-Binding Site. Biochem J 2005, 385 (Pt 1), 145–153. https://doi.org/10.1042/BJ20040773.
Schilling, O.; Späth, B.; Kostelecky, B.; Marchfelder, A.; Meyer-Klaucke, W.; Vogel, A. Exosite Modules Guide Substrate Recognition in the ZiPD/ElaC Protein Family. J Biol Chem 2005, 280 (18), 17857–17862. https://doi.org/10.1074/jbc.M500591200.
Marchfelder, A.; Hartmann, R. K. Highlight: RNA Biochemistry. Biol Chem 2005, 386 (12), 1203. https://doi.org/10.1515/BC.2005.138.
Dubrovsky, E. B.; Dubrovskaya, V. A.; Levinger, L.; Schiffer, S.; Marchfelder, A. Drosophila RNase Z Processes Mitochondrial and Nuclear Pre-tRNA 3’ Ends in Vivo. Nucleic Acids Res 2004, 32 (1), 255–262. https://doi.org/10.1093/nar/gkh182.
Schiffer, S.; Rösch, S.; Marchfelder, A. Recombinant RNase Z Does Not Recognize CCA as Part of the tRNA and Its Cleavage Efficieny Is Influenced by Acceptor Stem Length. Biol Chem 2003, 384 (3), 333–342. https://doi.org/10.1515/BC.2003.039.
Pellegrini, O.; Nezzar, J.; Marchfelder, A.; Putzer, H.; Condon, C. Endonucleolytic Processing of CCA-Less tRNA Precursors by RNase Z in Bacillus Subtilis. EMBO J 2003, 22 (17), 4534–4543. https://doi.org/10.1093/emboj/cdg435.
Kruszka, K.; Barneche, F.; Guyot, R.; Ailhas, J.; Meneau, I.; Schiffer, S.; Marchfelder, A.; Echeverría, M. Plant Dicistronic tRNA-snoRNA Genes: A New Mode of Expression of the Small Nucleolar RNAs Processed by RNase Z. EMBO J 2003, 22 (3), 621–632. https://doi.org/10.1093/emboj/cdg040.
Schiffer, S.; Rösch, S.; Marchfelder, A. Assigning a Function to a Conserved Group of Proteins: The tRNA 3’-Processing Enzymes. EMBO J 2002, 21 (11), 2769–2777. https://doi.org/10.1093/emboj/21.11.2769.
Schierling, K.; Rösch, S.; Rupprecht, R.; Schiffer, S.; Marchfelder, A. tRNA 3’ End Maturation in Archaea Has Eukaryotic Features: The RNase Z from Haloferax Volcanii. J Mol Biol 2002, 316 (4), 895–902. https://doi.org/10.1006/jmbi.2001.5395.
Schürer, H.; Schiffer, S.; Marchfelder, A.; Mörl, M. This Is the End: Processing, Editing and Repair at the tRNA 3’-Terminus. Biol Chem 2001, 382 (8), 1147–1156. https://doi.org/10.1515/BC.2001.144.
Schiffer, S.; Helm, M.; Théobald-Dietrich, A.; Giegé, R.; Marchfelder, A. The Plant tRNA 3’ Processing Enzyme Has a Broad Substrate Spectrum. Biochemistry 2001, 40 (28), 8264–8272. https://doi.org/10.1021/bi0101953.
Mörl, M.; Marchfelder, A. The Final Cut. The Importance of tRNA 3’-Processing. EMBO Rep 2001, 2 (1), 17–20. https://doi.org/10.1093/embo-reports/kve006.
Mayer, M.; Schiffer, S.; Marchfelder, A. tRNA 3’ Processing in Plants: Nuclear and Mitochondrial Activities Differ. Biochemistry 2000, 39 (8), 2096–2105. https://doi.org/10.1021/bi992253e.
Brennicke, A.; Marchfelder, A.; Binder, S. RNA Editing. FEMS Microbiol Rev 1999, 23 (3), 297–316. https://doi.org/10.1111/j.1574-6976.1999.tb00401.x.
Schock, I.; Maréchal-Drouard, L.; Marchfelder, A.; Binder, S. Processing of Plant Mitochondrial tRNAGly and tRNASer(GCU) Is Independent of RNA Editing. Mol Gen Genet 1998, 257 (5), 554–560. https://doi.org/10.1007/s004380050681.
Marchfelder, A.; Clayton, D. A.; Brennicke, A. The Gene for Ribosomal Protein L7a-1 in Schizosaccharomyces Pombe Contains an Intron after the Initiation Codon. Biochim Biophys Acta 1998, 1397 (2), 146–150. https://doi.org/10.1016/s0167-4781(98)00011-6.
Kunzmann, A.; Brennicke, A.; Marchfelder, A. 5’ End Maturation and RNA Editing Have to Precede tRNA 3’ Processing in Plant Mitochondria. Proc Natl Acad Sci U S A 1998, 95 (1), 108–113. https://doi.org/10.1073/pnas.95.1.108.
Marchfelder, A.; Brennicke, A.; Binder, S. RNA Editing Is Required for Efficient Excision of tRNA(Phe) from Precursors in Plant Mitochondria. J Biol Chem 1996, 271 (4), 1898–1903. https://doi.org/10.1074/jbc.271.4.1898.
Binder, S.; Marchfelder, A.; Brennicke, A. Regulation of Gene Expression in Plant Mitochondria. Plant Mol Biol 1996, 32 (1–2), 303–314. https://doi.org/10.1007/BF00039387.
Marchfelder, A. Plant Mitochondrial RNase P. Mol Biol Rep 1995, 22 (2–3), 151–156. https://doi.org/10.1007/BF00988721.
Marchfelder, A.; Brennicke, A. Characterization and Partial Purification of tRNA Processing Activities from Potato Mitochondria. Plant Physiol 1994, 105 (4), 1247–1254. https://doi.org/10.1104/pp.105.4.1247.
Binder, S.; Marchfelder, A.; Brennicke, A. RNA Editing of tRNA(Phe) and tRNA(Cys) in Mitochondria of Oenothera Berteriana Is Initiated in Precursor Molecules. Mol Gen Genet 1994, 244 (1), 67–74. https://doi.org/10.1007/BF00280188.
Marchfelder, A.; Brennicke, A. Plant Mitochondrial RNase P and E. Coli RNase P Have Different Substrate Specificities. Biochem Mol Biol Int 1993, 29 (4), 621–633.
Binder, S.; Marchfelder, A.; Brennicke, A.; Wissinger, B. RNA Editing in Trans-Splicing Intron Sequences of Nad2 mRNAs in Oenothera Mitochondria. J Biol Chem 1992, 267 (11), 7615–7623.
Marchfelder, A.; Schuster, W.; Brennicke, A. In Vitro Processing of Mitochondrial and Plastid Derived tRNA Precursors in a Plant Mitochondrial Extract. Nucleic Acids Res 1990, 18 (6), 1401–1406. https://doi.org/10.1093/nar/18.6.1401.