Detection of Antigen-Specific T Cells using Multiparameter Flow Cytometry

Duration: 2 Days
Venue: Project Team Böhm, Department of Internal Medicine I
Participants: min. 1, max. 3
Info: Detection of antigen-specific T cells is usually quite difficult due to the little amount of these cells in the peripheral blood (less than 0.1% of lymphocytes). Within this course the multicolour FACS analysis will be used to determine both phenotypic (expression of activation markers) and functional (cytokine secretion) characteristics of such rare cell populations. The primary advantage of this technique is that many distinct measurements can be performed simultaneously on one sample.
ECTS Credits: 5
Contact: Dr. Silke Rosinger, Tel.: 500-44515

Nuclear extracts - Western Blot - analysis in type2 diabestes mellitus 

Duration: 2 Days
Venue: Project Team Böhm, Department of Internal Medicine I
Participants: min. 1, max. 3
Info: 
ECTS Credits: 5
Contact: Fang Zou, Tel.: 500-44515

Immunstaining and fluorescence microscopy

Duration: 2-3 Days
Venue: Project Team P4 - Wichert, Department of Internal Medicine I 
Participants: min. 1, max. 2

Info: The expression and localisation of proteins in cells can be studied by immunostaining and further analysed by fluorescence microscopy. In this course we perform the sample preparation and give an introduction to fluorescence microscopy for interested students. It is possible to bring your own samples or cells for this course.
ECTS Credits: 5
Contact: Christin Münzberg, Tel.: 500-44605

Expression and detection of different preproinsulin mutants in transiently transfected, non-pancreatic cells

Duration: 2-3 Days
Venue:  Project Team P2 - Schirmbeck, Department of Internal Medicine I
Participants:

Info: Transient transfection of HEK cells; immunoprecipitation; western blotting; 
ECTS Credits: 5
Contact: Prof. Dr. R. Schirmbeck, Tel.: 500-44685

Adipogenic differentiation of human mesenchymal precursor cells

Duration: 3 Days (flexible scheduling)
Venue:  Project Team P5 - Wabitsch, Klinik für Kinder- und Jugendmedizin
Participants:

Info: Cell culture, determination of adipogenic differentiation rate and trigylceride content, qPCR of adipocyte-specific marker genes;
ECTS Credits: 5
Contact: Dr. P. Fischer-Posovszky, Tel.: 500-57415