This project will develop transporter molecules for efficient delivery of bioactive peptides into specific target cell types or across the blood-brain-barrier. To achieve this, non-toxic mutants of bacterial AB-type protein toxins will be generated and used that mediate cell-targeting by specific receptor-binding subunits and controlled release of the cargo peptides into the cytosol by specific translocation subunits. These processes will be analyzed in living cells by state-of-the-art imaging methods developed in this project such as lattice light sheet microscopy and single molecule tracking. The techniques will be applied to antimicrobial and anticancer peptides which were examined within this CRC but are of general interest for potent and specific delivery.

 

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