Z3: The core unit mass spectrometry and proteomics

This central project will continue to provide means to various groups within CRC 1074 to use state-of-the-art mass spectrometry-based proteomics. Z3 offers qualitative and quantitative proteomics to understanding the dynamics involved in leukemic processes. In addition, due to their importance in cellular processes, means for the analyses of various post-translational modifications (PTM), including phosphorylation, glycosylation, ubiquitination and SUMOylation are provided. Furthermore, Z3 allows for proteoform characterization, thus enabling projects within the CRC to understand intra-molecular crosstalk between PTMs. Z3 will develop methods to allow for proteomic analyses of samples with very low cell numbers as generated during FACS-based cell sorting. Furthermore, after having established chemical cross-linking, projects will be able to characterize binding sites within and between protein complexes. Employing thermal proteome profiling offers projects the ability to search for unknown drug interactions on a proteome wide scale.

For a current list of all project-related publications, please go to this page