The Core Facility Light Microscopy is a member of the Centre for Translational Imaging - From Molecule to Man (MoMAN). The facility can be accessed by all scientists of the Medical Faculty and Ulm University and offers a wide spectrum of optical microscopy approaches. Our main technique, confocal microscopy, is based on the pixelwise scanning with illumination lasers to generate virtual 2D optical sections through 3D objects.

The core facility primarily provides training that allows users to later operate our microscopes independently. In addition, we offer support with the evaluation and interpretation of the image data, in particular concerning quantitative and statistical image analysis. Due to our long standing expertise we can also offer help with the design of imaging based experiments and the development of bespoke experimental protocols.

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• Inverse confocal microscope.
• Equipped with sensitive hybrid detectors.
• Optimized for quantiative fluorescence imaging.
• Equipped with a stage incubator for live cell imaging.
• Laser lines 405, 488, 561 and 633 nm.

• Inverse confocal microscope
• Equipped with TCSPC detectors and a Spectra Physics MaiTai IR-Laser for pulsed excitation
• Optimized for time correlated single photon counting applications such as FLIM (fluorescence lifetime imaging) or FLIM/FRET, e.g. for metabolic imaging
• Multiphoton and SHG (second harmonic generation) - microscopy
• Equipped with incubators for live cell imaging
• Laser lines 405, 488, 561 and 633 nm.

Facility services include:

• Standard and multiphoton confocal microscopy
• Live cell imaging
• Time resolved techniques such as FLIM, FLIM/FRET or FCCS
• Expertise in experiment design, staining protocols, subcellular imaging, …
• Support with quantitative image analysis and image statistics
• Hands on support for complex imaging tasks